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human breast carcinoma mcf 7 cells  (ATCC)


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    Structured Review

    ATCC human breast carcinoma mcf 7 cells
    Human Breast Carcinoma Mcf 7 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 34878 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+carcinoma+cells+mcf+7/MCF7/pmc13179769-74-5-16
    Average 99 stars, based on 34878 article reviews
    human breast carcinoma mcf 7 cells - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Exploring variations in glycolytic and gluconeogenic enzymes and isoforms across breast cancer cell lines and tissues.
    Article Snippet: Non-tumorigenic epithelial MCF-10A cell line (ATCC CRL-10317, purchased to American Tissue and Cell Culture, USA), was cultured in DMEM/F12 medium (Gibco Life technologies, Netherlands) containing 1 % penicillin/streptomycin (Invitrogen Life technologies, USA), 20 ng/ mL epidermal growth factor (Sigma-Aldrich, USA), 100 ng/mL Cholera toxin (Sigma–Aldrich, USA), 10 μg/mL insulin (Sigma-Aldrich, USA), 0.5 mg/mL hydrocortisone (Sigma-Aldrich, USA), and 10 % horse serum (Pan Biotech, Germany). .. Human breast carcinoma cells MCF-7 (ATCC HTB22, purchased to American Tissue and Cell Culture, USA) were cultured in DMEM high glucose (Sigma–Aldrich, USA), supplemented with 1 % penicillin/ streptomycin (Invitrogen Life technologies, USA), 3.7 g/L sodium bicarbonate (Merck, Germany), and 10 % Fetal Bovine Serum (Invitrogen Life technologies, USA). ..

    Article Title: Synthesis, antitumor activity and structure-activity studies of novel pyridoxine-based bioisosteric analogs of estradiol.
    Article Snippet: A new efficient approach to the synthesis of 6-alkenyl substituted pyridoxine derivatives has been developed.. A series of 31 novel alkenyl pyridoxine derivatives, stilbene-based bioisosteric analogs of estradiol, were synthesized.. In vitro cytotoxicity of the obtained compounds against MCF-7 (ER+) breast cancer tumor cells was studied using the MTT assay.

    Article Title: A new indoloquinoline-based fluorescence probe for imaging of hydrazine in vitro and in vivo
    Article Snippet: .. The human breast carcinoma cells MCF-7 (ATCC, USA), and normal breast epithelial cells MCF-10A (CCTCC, China), were cultured under controlled conditions. ..

    Article Title: A Versatile Nanoplatform Based on Metal‐Phenolic Networks Inhibiting Tumor Growth and Metastasis by Combined Starvation/Chemodynamic/Immunotherapy
    Article Snippet: Although much progress has been made by multifunctional nanoplatforms in the treatment of cancer, several defects of existing nanoplatforms, such as tedious preparation, poor biocompatibility, and failure to activate the immune system, have limited their clinical applications.. Herein, a versatile nanosystem of folic acid-modified metal-phenolic networks (MPNs) loaded with GOx and CHA (F-MGC) is fabricated by the easy self-assembly of MPNs, during which glucose oxidase (GOx) and chlorogenic acid (CHA) are concurrently loaded.. The resulting nanosystem, having a folic acid-modified surface and inherent acid sensitivity, shows versatility in being able to target tumors and release active ingredients in the weakly acidic tumor microenvironment (TME).

    Article Title: The secretome of non-tumorigenic mammary cells MCF-10A elicits DNA damage in MCF-7 and MDA-MB-231 breast cancer cells.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Multiple Displacement Amplification:

    Article Title: Synthesis, antitumor activity and structure-activity studies of novel pyridoxine-based bioisosteric analogs of estradiol.
    Article Snippet: A new efficient approach to the synthesis of 6-alkenyl substituted pyridoxine derivatives has been developed.. A series of 31 novel alkenyl pyridoxine derivatives, stilbene-based bioisosteric analogs of estradiol, were synthesized.. In vitro cytotoxicity of the obtained compounds against MCF-7 (ER+) breast cancer tumor cells was studied using the MTT assay.

    Article Title: Non-tumorigenic epithelial breast cells and ionizing radiation cooperate in the enhancement of mesenchymal traits in tumorigenic breast cancer cells.
    Article Snippet: Aims: Radioresistance and recurrences are crucial hindrances in cancer radiotherapy.. Fractionated irradiation can elicit a mesenchymal phenotype in irradiated surviving cells and a deep connection exists between epithelial mesenchymal transition, radioresistance, and metastasis.. The aim of this study was to analyze the effect of the secretoma of irradiated non-tumorigenic mammary epithelial cells on surviving irradiated breast tumor cells regarding the gain of mesenchymal traits and migratory ability.

    Article Title: The secretome of non-tumorigenic mammary cells MCF-10A elicits DNA damage in MCF-7 and MDA-MB-231 breast cancer cells.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Isolation:

    Article Title: Synthesis, antitumor activity and structure-activity studies of novel pyridoxine-based bioisosteric analogs of estradiol.
    Article Snippet: A new efficient approach to the synthesis of 6-alkenyl substituted pyridoxine derivatives has been developed.. A series of 31 novel alkenyl pyridoxine derivatives, stilbene-based bioisosteric analogs of estradiol, were synthesized.. In vitro cytotoxicity of the obtained compounds against MCF-7 (ER+) breast cancer tumor cells was studied using the MTT assay.



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    In vitro biocompatibility evaluation of probe 1 after incubation with MCF-10A cells (A) <t>and</t> <t>MCF-7</t> (B) cells.
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    In vitro biocompatibility evaluation of probe 1 after incubation with MCF-10A cells (A) <t>and</t> <t>MCF-7</t> (B) cells.
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    A) Schematic diagram of the experimental design for assessing the motility of Raw264.7 cells after metabolic intervention or hypoxia induction in vitro using living cell imaging technology. B) Representative images and migration tracks of Raw264.7 cells under different treatments. C) Average intracellular ATP content ( n = 3), and D) cell migration velocity under different treatment conditions ( n = 5). E) CLSM images of hypoxia levels in 4T1 <t>and</t> <t>MCF‐7</t> cells marked with hypoxia probes. F) CLSM images of HIF‐1α expression in 4T1 and MCF‐7 cells labeled by immunofluorescence staining. G,H) Semi‐quantitative analysis of hypoxia levels and HIF‐1α expression by fluorescence intensity ( n = 3). Data are presented as mean ± SD. Statistical significance was calculated via one‐way ANOVA with Tukey’ post hoc test. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
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    A) Schematic diagram of the experimental design for assessing the motility of Raw264.7 cells after metabolic intervention or hypoxia induction in vitro using living cell imaging technology. B) Representative images and migration tracks of Raw264.7 cells under different treatments. C) Average intracellular ATP content ( n = 3), and D) cell migration velocity under different treatment conditions ( n = 5). E) CLSM images of hypoxia levels in 4T1 <t>and</t> <t>MCF‐7</t> cells marked with hypoxia probes. F) CLSM images of HIF‐1α expression in 4T1 and MCF‐7 cells labeled by immunofluorescence staining. G,H) Semi‐quantitative analysis of hypoxia levels and HIF‐1α expression by fluorescence intensity ( n = 3). Data are presented as mean ± SD. Statistical significance was calculated via one‐way ANOVA with Tukey’ post hoc test. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
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    A) Schematic diagram of the experimental design for assessing the motility of Raw264.7 cells after metabolic intervention or hypoxia induction in vitro using living cell imaging technology. B) Representative images and migration tracks of Raw264.7 cells under different treatments. C) Average intracellular ATP content ( n = 3), and D) cell migration velocity under different treatment conditions ( n = 5). E) CLSM images of hypoxia levels in 4T1 <t>and</t> <t>MCF‐7</t> cells marked with hypoxia probes. F) CLSM images of HIF‐1α expression in 4T1 and MCF‐7 cells labeled by immunofluorescence staining. G,H) Semi‐quantitative analysis of hypoxia levels and HIF‐1α expression by fluorescence intensity ( n = 3). Data are presented as mean ± SD. Statistical significance was calculated via one‐way ANOVA with Tukey’ post hoc test. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
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    Image Search Results


    In vitro biocompatibility evaluation of probe 1 after incubation with MCF-10A cells (A) and MCF-7 (B) cells.

    Journal: RSC Advances

    Article Title: A new indoloquinoline-based fluorescence probe for imaging of hydrazine in vitro and in vivo

    doi: 10.1039/d5ra08919k

    Figure Lengend Snippet: In vitro biocompatibility evaluation of probe 1 after incubation with MCF-10A cells (A) and MCF-7 (B) cells.

    Article Snippet: The human breast carcinoma cells MCF-7 (ATCC, USA), and normal breast epithelial cells MCF-10A (CCTCC, China), were cultured under controlled conditions.

    Techniques: In Vitro, Incubation

    Fluorescence imaging of probe 1 in MCF-7 tumor-bearing mice at various intervals pre- and post-injection.

    Journal: RSC Advances

    Article Title: A new indoloquinoline-based fluorescence probe for imaging of hydrazine in vitro and in vivo

    doi: 10.1039/d5ra08919k

    Figure Lengend Snippet: Fluorescence imaging of probe 1 in MCF-7 tumor-bearing mice at various intervals pre- and post-injection.

    Article Snippet: The human breast carcinoma cells MCF-7 (ATCC, USA), and normal breast epithelial cells MCF-10A (CCTCC, China), were cultured under controlled conditions.

    Techniques: Fluorescence, Imaging, Injection

    A) Schematic diagram of the experimental design for assessing the motility of Raw264.7 cells after metabolic intervention or hypoxia induction in vitro using living cell imaging technology. B) Representative images and migration tracks of Raw264.7 cells under different treatments. C) Average intracellular ATP content ( n = 3), and D) cell migration velocity under different treatment conditions ( n = 5). E) CLSM images of hypoxia levels in 4T1 and MCF‐7 cells marked with hypoxia probes. F) CLSM images of HIF‐1α expression in 4T1 and MCF‐7 cells labeled by immunofluorescence staining. G,H) Semi‐quantitative analysis of hypoxia levels and HIF‐1α expression by fluorescence intensity ( n = 3). Data are presented as mean ± SD. Statistical significance was calculated via one‐way ANOVA with Tukey’ post hoc test. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Journal: Advanced Science

    Article Title: Photo‐Empowered Macrophage‐Based Drug Delivery System Overcomes Motility Suppression and Significantly Enhances Deep Tumor Drug Delivery

    doi: 10.1002/advs.202515349

    Figure Lengend Snippet: A) Schematic diagram of the experimental design for assessing the motility of Raw264.7 cells after metabolic intervention or hypoxia induction in vitro using living cell imaging technology. B) Representative images and migration tracks of Raw264.7 cells under different treatments. C) Average intracellular ATP content ( n = 3), and D) cell migration velocity under different treatment conditions ( n = 5). E) CLSM images of hypoxia levels in 4T1 and MCF‐7 cells marked with hypoxia probes. F) CLSM images of HIF‐1α expression in 4T1 and MCF‐7 cells labeled by immunofluorescence staining. G,H) Semi‐quantitative analysis of hypoxia levels and HIF‐1α expression by fluorescence intensity ( n = 3). Data are presented as mean ± SD. Statistical significance was calculated via one‐way ANOVA with Tukey’ post hoc test. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Article Snippet: Mouse breast carcinoma cells 4T1 (RRID: CVCL_0125), human breast carcinoma cells MCF‐7 (RRID: CVCL_0031), and mouse monocyte‐macrophage cells RAW264.7 (RRID: CVCL_0493) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China) in February 2023.

    Techniques: In Vitro, Imaging, Migration, Expressing, Labeling, Immunofluorescence, Staining, Fluorescence